Chemiluminescence method for the assay of perphenazine in drug formulation using permanganate in sulphuric acid with flow injection technique and a chemometrical optimization approach

摘要

An accurate selective flow injection chemiluminescence (CL) method for the assay of perphenazine was explored. In the method 394 ppm permanganate solution was used as a chemiluminogenic reagent in 0.289 mol dm~(-3) sulphuric acid media. A photomultiplier tube was used as a detector at a total flow rare of 4.94 ml/min. Perphenazine was determined by a linear calibration plot of the following equation in the range 50-350 ppm: mV = -4.488 + 0.1162C, with a correlation coefficient of 0.9989 for five measurements and a relative standard deviation less than 2.33. A sampling frequency not less than 110 samples h~(-1) was established. Three factors namely, the flow rate, sulphuric acid and permanganate concentrations were found to have an influence on the amount of chemiluminescence intensity produced. Therefore, their interaction effects were thoroughly investigated by employing the 2~3 factorial design chemometrical approach and the results obtained revealed a higher interaction between sulphuric acid and permanganate and a less significant interaction for both reagents with the flow rate. The interaction of variables observed necessitated the conduct of the super modified simplex optimization procedure which has resulted in offering the proper optimum conditions as stated and led to the quantitative assay of perphenazine. An interference study indicated that the method was suitable for application in pharmaceutical preparations.