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Biologic variability of sperm DNA denaturation in infertile men.

机译:不育男性精子DNA变性的生物学变异性。

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OBJECTIVES: To examine sperm DNA denaturation (DD) in fertile and infertile men and assess the variability of conventional semen parameters and sperm DD in repeated semen samples from infertile men. METHODS: Twenty-one consecutive nonazoospermic, infertile men each submitted two semen samples, 2 to 6 weeks apart. We examined semen samples from consecutive fertile men (n = 10) presenting for vasectomy as controls. Standard semen parameters (World Health Organization criteria) and sperm chromatin structure (evaluated by flow cytometry analysis of acridine orange-treated spermatozoa and expressed as the percentage of spermatozoa with denatured DNA) were monitored. RESULTS: Fertile men had a significantly higher sperm concentration and percentage of sperm motility and a significantly lower percentage of sperm with DD than did infertile men (36 +/- 5.2 x 10(6)/mL versus 12.5 +/- 2.2 x 10(6)/mL, 60.0% +/- 5.2% versus 30.1% +/- 4.1%, and 8.9% +/- 1.9% versus 20.3% +/- 2.5%, respectively, P <0.05). The sperm concentration, sperm motility, and percentage of spermatozoa with DD were not significantly different between the first and second semen samples from the infertile men. Sperm DD showed the lowest average within-subject coefficient of variation (SD/mean), followed by motility and concentration (coefficient of variation 21%, 24%, and 35%, respectively). CONCLUSIONS: Our data demonstrate that infertile men have significantly higher sperm DD compared with fertile men and that sperm DD exhibits a low coefficient of variation ( approximately 20%) on repeated assessment. These data suggest that sperm DD has a relatively low degree of biologic variability.
机译:目的:检查可育和不育男性的精子DNA变性(DD),并评估不育男性重复精液样本中常规精液参数和精子DD的变异性。方法:21名连续的非无精子症,不育男性各提交了两个精液样本,相隔2至6周。我们检查了来自进行输精管结扎术的连续可育男性(n = 10)的精液样本。监测标准精液参数(世界卫生组织标准)和精子染色质结构(通过flow啶橙处理的精子的流式细胞术分析评估,并表示为具有变性DNA的精子的百分比)。结果:与不育男性相比,可育男性的精子浓度和精子活力百分比显着更高,DD精子百分比显着更低(36 +/- 5.2 x 10(6)/ mL对12.5 +/- 2.2 x 10( 6)/ mL,分别为60.0%+/- 5.2%与30.1%+/- 4.1%和8.9%+/- 1.9%与20.3%+/- 2.5%,P <0.05)。在不育男性的第一和第二精液样本中,DD的精子浓度,精子运动力和精子百分率没有显着差异。精子DD的平均受试者内部变异系数(SD /平均值)最低,其次是运动力和浓度(变异系数分别为21%,24%和35%)。结论:我们的数据表明,不育男人的精子DD明显高于可育男人,并且经反复评估,精子DD的变异系数较低(约20%)。这些数据表明,精子DD具有相对较低的生物学变异性。

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