SOMATIC EMBRYOGENESIS AND PLANT REGENERATION OF TIGRIDIA PAVONIA (LF) DC., A MEXICAN NATIVE ORNAMENTAL PLANT

摘要

Tigridia pavonia (L.f.) DC., also known as "tiger flower" is reproduced by seed or by bulb. However, when it is propagated by seed the germination rates are very low and in order to get the mature bulbs it takes at least three years. In the present study, somatic embryos formation was induced through embryogenic callus cultivated on half-strenth Litvay et al. medium supplemented with 0.5 g l(-1) L-glutamine and 1 mg l(-1) abscisic acid enhanced maturation of somatic embryos. The histological analysis confirmed the unicellular origin of somatic embryos showing the presence of procambium in the globular stage, and vascular tissue in the somatic embryos completely differentiated. The protocol developed allowed us to obtain 10.9 plants per explant.