Isolation and Characterization of a Novel Small Antifungal Peptide from Bacillus megaterium D4 Isolated from the Dung of Wild Plateau Yak in China

摘要

A novel small antifungal peptide-producing strain D4 was isolated from the dung of wild plateau yak and identified as Bacillus megaterium. The purification procedure of peptide consisted of acid precipitation, methanol extract and C-18 reverse-phase chromatography. The amino acid composition of peptide YP differed from those of antifungal peptides which have been reported to date. Peptide YP was proved to be a novel small antifungal cyclic peptide. It exhibited strong inhibitory activity against many phytopathogenic fungi and had a wild range of thermo stability and pH stability and was not susceptible to all proteases tested.