Analysis of expression of GAPDH gene in Solanum lycopersicum L infected with Tomato torrodo virus (ToTV)

摘要

Real-time PCR (polymerase chain reaction) is a method commonly used for analysis of genes expression. However, accurate interpretation of real-time PCR results needs additional normalization step, where the expression level of analyzed gene is corrected to the actual amount of total RNA taken for the analysis. As a normalizer any gene can be used, provided its expression is stable during the experiment. GAPDH (glyceraldehyde 3-phosphate dehydrogenase) is one of candidates that might be used as a reference gene for normalization of real-time PCR. Here we indicated a high identity of GAPDH mRNA sequence isolated from ten varieties of tomato {Solanum lycopersicum). Moreover, we reported here, that expression of the gene was rather unstable in tomato during Tomato torrado virus infection (ToTV).