Occurrence of the white potato nematode Globodera pallida (Stone, 1973) Behrens, 1975 (Nematoda: Heteroderidae) on the territory of Poland

摘要

In June 2010, the presence of 40 cysts of nematodes from the Globodera genus was revealed in a soil sample collected from the field in the area of Opolskie Voivodeship in Poland. The results of the morphological and morphometric analyses revealed thepresence of 30 cysts with features characteristic for the species Globodera rostochiensis (Wollenweber, 1923) Skarbilovich, 1959, and 10 cysts with features characteristic for the species G. pallida (Stone, 1973) Behrens, 1975. The results of molecular tests (PCR -Polymerase Chain Reaction and PCR-RFLP - Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) confirmed the presence of both nematode species in the sample. Sequence of rDNA fragment (ribosomal deoxyribonucleic acid) obtained inthe PCR amplification and comparative analysis of the sequence revealed approximately 97% identity with a sequence characteristic for G. pallida deposited in the NCBI database (National Center for Biotechnology Information), which confirmed the presenceof this species in the sample.