Polyethylene Glycol-Containing Supports for Solid-Phase Synthesis of Peptides and Combinatorial Organic Libraries

摘要

The choice of polymeric support is a key factor for the success of solid-phase methods for syntheses of organic compounds and biomolecules such as peptides and oligonucleotides. Classical Merrifield solid-phase peptide synthesis (SPPS), performed on low cross-linked hydrophobic polystyrene (PS) beads, sometimes suffers from sequence-dependent coupling difficulties. More hydrophilic polyamide supports were developed to overcome these problems4. However, neither PS nor amide supports are suitable for continuous-flow applications. This lecture reviews the preparation of families of polyethylene glycol-polystyrene (PEG-PS) graft5'7 and Cross-JUnked Ethoxylate Acrylate Resin (CLEAR)8 supports, and demonstrates their applications to synthesis.