CONTENT OF PHENOLIC COMPOUNDS IN HUMUS ACIDS OF FOREST SOIL

摘要

The aim of the present paper was to define the qualitative and quantitative composition of phenolic compounds in the extracts of fulvic acids and hydrolyzates of humic acids isolated from forest soil. Forest soil was sampled from the organic horizon (5-3.5 cm - sub-horizon Ol and 3.5-0 cm - sub-horizon Ofh) and mineral horizon every 10 cm (0-50 cm). Phenolic compounds in fulvic acids (FAs) extracts and humic acids (HAs) hydrolyzates were identified by high performance liquid chromatography (HPLC). The recorded chromatograms showed differences in the composition of phenolic compounds between humic and fulvic acids fractions. The content of those compounds both in extracts of fulvic acids and hydrolyzates of humic acids and V+S+C parameter values decreased together with the sampling depth. The highest values of V+S+C parameter were calculated for extracts of fulvic acids and humic acids hydrolyzates isolated from the organic horizon of forest soil. The share of the vanillyl type in the extracts of humus acids (HAs+FAs) of the subhorizon Ol was close to that of the sum share of the syringyl and cinnamyl type. The greater the depth, the greater the share of vanillyl compounds in the pool of the share of syringyl, cinnamyl and vanillyl compounds.