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首页> 外文期刊>Plant molecular biology reporter >Differential Impact of Acclimation and Acute Water Deprivation in the Expression of Key Transcription Factors in Soybean Roots
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Differential Impact of Acclimation and Acute Water Deprivation in the Expression of Key Transcription Factors in Soybean Roots

机译:驯化和急性缺水对大豆根系关键转录因子表达的不同影响

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摘要

Soybean (Glycine max) is one of the major world commodities. In order to increase the soybean yields, it has been searched drought-tolerant cultivars, once the drought is the major constraint to soybean grown. Therefore, it is crucial to elucidate the molecular mechanisms associated with drought tolerance. Here, the in silico approach allowed us to identify 12 genes belonging to six different transcription factor families in soybean that have been associated with key events on drought response. The expression pattern of each gene was investigated by qPCR in root samples of drought-sensitive and drought-tolerant cultivars undergoing drought stress in pot-based (PSys) and hydroponic (HSys) cultivation systems. GmaxMYC2-like 2 was induced under abrupt drought conditions in HSys in both cultivars, whereas GmaxAREB1-like 1 and GmaxDREB2A-like were highly induced only in the PSys. However, GmaxMYB2-like 1, GmaxMYB2-like 2, GmaxRD26/NAC-like 1, GmaxRD26/NAC-like 2, GmaxAREB1-like 2, and GmaxDREB1A-like were upregulated in both systems, while the GmaxHB6-like and GmaxHB13-like are repressed under all of the investigated conditions. Exogenous abscisic acid (ABA) treatment was used to identify those genes belonging to the ABA-dependent drought response. The genes identified in this work have potential application for the improvement of drought resistance in soybean and markers for breeding programs.
机译:大豆(Glycine max)是世界主要商品之一。为了增加大豆产量,一旦干旱是限制大豆生长的主要因素,就已经研究了耐旱品种。因此,阐明与干旱耐受性相关的分子机制至关重要。在这里,计算机方法使我们能够鉴定属于大豆中六个不同转录因子家族的12个基因,这些基因与干旱响应的关键事件有关。通过qPCR研究在盆栽(PSys)和水培(HSys)栽培系统中遭受干旱胁迫的干旱敏感和耐旱品种的根样品中每个基因的表达模式。在两个品种的HSys中,在干旱条件下均诱导了GmaxMYC2-like 2,而仅在PSys中高度诱导了GmaxAREB1-like 1和GmaxDREB2A-like。然而,在两个系统中,类GmaxMYB2 1,类GmaxMYB2 2,类GmaxRD26 / NAC 1,类GmaxRD26 / NAC 2,类GmaxAREB1 2和类GmaxDREB1A均上调,而类GmaxHB6和GmaxHB13类。在所有调查的条件下都被抑制。外源脱落酸(ABA)处理用于鉴定属于ABA依赖干旱反应的那些基因。这项工作中鉴定出的基因对于提高大豆的抗旱性和育种标记具有潜在的应用价值。

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