Effect of aluminum on the isozymes of the seedlings of two soybeans [Glycine max (L.) Merrill] varieties.

摘要

Laboratory experiments were conducted to analyse the isoenzymes of seedlings of the soyabean cultivars Zhechun No. 3 and Huachun No. 18 and their response to different aluminium (Al) treatments. Results showed that the responses to Al toxicity of different isoenzymes were quite different. Among the isoenzymes, esterase (EST) was the least sensitive to Al toxicity. There was no distinct difference in the zymograms of EST under different Al treatments. Al toxicity enhanced the activity of peroxidase (POD) and induced some new bands to adapt it. Superoxide dismutase (SOD) was inhibited by Al toxicity and showed a decrease in activity and disappearance of some bands. The cytochrome-oxidase (cytochrome-c oxidase; COD) activity was also enhanced, but the enhancement of the activity of POD and COD occurred under the treatments with low Al concentrations. An increase in the Al concentration disturbed the expression of POD and COD, which showed that the activity of those isoenzymes decreased. Furthermore, there was no consistency with the response to Al treatments among different bands of the same isoenzymes. Besides, there was some genotypic variation between 2 soyabean cultivars. The bands of these isoenzymes varied differently between these 2 soyabean cultivars under the same Al treatments. Compared to Huachun No. 18, Zhechun No. 3 had a relatively stronger tolerance to Al stress..