The Methods of Quantitative Proteomics

摘要

In modern science proteomic analysis is inseparable from other fields of systemic biology. Possess-ing huge resources quantitative proteomics operates colossal information on molecular mechanisms of life.Advances in proteomics help researchers to solve complex problems of cell signaling, posttranslational modi-fication, structure and funciotnal homology of proteins, molecular diagnostics etc. More than 40 various meth-ods have been developed in proteomics for quantitative analysis of proteins. Although each method is uniqueand has certain advantages and disadvantages all these use various isotope labels (tags). In this review we willconsider the most popular and effective methods employing both chemical modifications of proteins and alsometabolic and enzymatic methods of isotope labeling.