(+)-Abscisic acid metabolism, 3-ketoacyl-coenzyme A synthase gene expression, and very-long-chain monounsaturated fatty acid biosynthesis in Brassica napus embryos

摘要

Microspore-derived embryoids of Brassica napus (rape) cv. Reston were used to examine the effects of exogenous (+)-abscisic acid (ABA) and related compounds on the accumulation of very-long-chain monounsaturated fatty acids (VLCMFAs), VLCMFA elongase complex activity, and induction of the 3-ketoacyl-coenzyme A synthase (KCS) gene encoding the condensing enzyme of the VLCMFA elongation system. Of the concentrations tested, (+)-ABA at 10μM showed the strongest effect. Maximum activity of the elongase complex, observed 6 h after 10μM (+)-ABA treatment, was 60% higher than that of the untreated embryoids at 24 h. The transcript of the KCS gene was induced by 10μM (+)-ABA within 1 h and further increased up to 6 h. The VLCMFAs eicosenoic acid (20:1)and erucoic acid (22:1) increased by 1.5- to 2-fold in embryoids treated with (+)-ABA for 72 h. Also, (+)-8'-methylene ABA, which is metabolized more slowly than ABA, had a stronger ABA-like effect on the KCS gene transcription, elongase complex activity (28% higher), and level of VLCMFAs (25-30% higher) than ABA. After 24 h approximately 60% of the added (+)-[3H]ABA (10μM) was metabolized, yielding labelled phaseic and dihydrophaseic acid. It is concluded that (+)-ABA promotes VLCMFA biosynthesis viaincreased expression of the KCS gene and that reducing ABA catabolism would increase VLCMFAs in microspore-derived embryoids.