Response characteristics of seed germination and seedling growth of Acorus tatarinowii under diesel stress.

摘要

Aims: Responses of typical wetland plant Acorus tatarinowii to diesel stress were investigated to provide basis of ecological monitoring system and phytoremediation for diesel-contaminated wetland. Methods: Greenhouse experiments were established to determine the germinability of seedlings, hydrogen peroxide in leaves, and DNA damage in roots exposed to a range of potentially phytotoxic diesel. Results: The presence of diesel did not benefit the growth of A. tatarinowii. The germination ratio and germination rate decreased with the increase of diesel concentration, both the lowest value appeared when the concentration of diesel was 10,000 mg kg-1. The lowest diesel concentration (2,000 mg kg-1) in the soil significantly reduced the length, average diameter, and projected area of root, especially on the stress of the higher diesel concentration (4,000, 8,000, and 10,000 mg kg-1). Furthermore, H₂O₂ concentration in leaves rose with the increasing concentration of diesel. However, no DNA oxidative damage to root was observed in our experiment. Conclusions: Diesel exposure significantly inhabited the seed germination, root elongation, and seedlings growth of A. tatarinowii. Diesel stress caused the accumulation of H₂O₂ in the leaves of A. tatarinowii.