GENETICS OF ALDEHYDE DEHYDROGENASE ISOZYMES IN THE MOUSE: EVIDENCE FOR MULTIPLE LOCI AND LOCALIZATION OF Ahd-2 ON CHROMOSOME 19

摘要

Electrophoretic and activity variation of the cytoplasmic isozyme of aldehyde dehydrogenase (designated AHD-B4) was observed among inbred strains and Harwell linkage-testing stocks of Mus musculus . The phenotypes are inherited in a normal Mendelian fashion, with two alleles showing co-dominant expression at a single locus ( Ahd-2 ). The locus was shown to segregate independently of Ahd-1 (encoding the mitochondrial AHD-A2 isozymes on chromosome 4 ; Holmes 1978). Linkage data of Ahd-2 with ep (pale ears), ru (ruby eyes) and bm (brachymorphic) suggest that it is localized near the centromeric end of chromosome 19 . Electrophoretic evidence for a third AHD isozyme (designated AHD-Cy), which is predominantly localized in the liver microsomal fraction, is also presented.