Utility of DNA Barcoding Tool for Conservation and Molecular Identification of Intraspecies of Rice Genotypes Belonging to Chhattisgarh Using RBCL and MATK Gene Sequences

摘要

India is endowed with a great diversity of rice germplasm, among different state Chhattisgarh is well known for rice cultivation and called “rice bowl” of India. This genetic diversity among and between landraces, exists a wide scope for future crop improvement. In recent years, DNA barcoding has been suggested as a useful molecular technique to complement traditional taxonomic expertise for fast species identification and biodiversity inventories. In this study, in situ application of DNA barcodes was tested on selected diverse rice genotypes with the aim of contributing to the identification, conservation and protection of Intellectual Property Rights (IPR) of state. DNA Barcoding technique was successfully pioneered in animals using a portion of the cytochrome oxidase 1 (CO1) mitochondrial gene. In plants, establishing a standardized DNA barcoding system has been more challenging. Thus potential of the rbcl and matK markers for the selection of barcoding loci of rice genotypes. The panel of 231 diverse rice genotypes including germplasm lines, elite, varieties and wild rice were used. Our finding showed that amplification efficiency observed in panel of intraspecies of rice was in rbcl (89.6%) and matK (62.33%).On the basis of amplification efficiency panel of 24 rice genotypes selected for sequencing. The parsimony informative sites was estimated with maximum 305 sites recorded in Matk, followed by 264 sites Rbcl and number of variable sites reported highest in rbcl 672 followed by MatK-f 246.While nucleotide diversity per site pi(π) reported maximum in rbcl 0.21613(MEGA 7.025).This scientific information data submitted to Barcoding of life database (BOLD) for generation of illustrative barcode.