Using DNA and Isozyme Markers to Study Genetic Relationship Among High Regenerative Interspecific Hybrids of Helianthus Eggertii small. X Helianthus Annuus L.

摘要

RAPD, AP-PCR, IFLP, SSR and isozymes markers were used to characterize high and low regenerative beckcross lines and their parents?￠????Helianthus eggertii and Helianthus annuus. Thirty?￠????eight markers specific to Helianthus eggertii were developed. Applying data from DNA and isozymes analysis was studded relationships among lines using generated dendrogram by neighbor-joining method. The results demonstrated a possibility to using the dendrogram as methodology for early estimation of advantageous genotypes in plant selection for high regeneration potential.