Continuous enzymatic treatment of 4-bromophenol initiated by UV irradiation

摘要

Horseradish peroxidase (HRP) can be used for the treatment of halogenated phenolic substances.nIn the presence of hydrogen peroxide phenols are oxidized to form polymers which undergonpartial dehalogenation. However, when immobilized, the peroxidase is subject to inactivation duento blockage of the active sites by the growing polymers and to deactivation by elevated levels ofnhydrogen peroxide. When HRP immobilized on a novel glass-based support incorporating titaniumndioxide is subjected to UV irradiation, hydrogen peroxide is produced and the nascent polymernis removed. In this work a reactor was constructed that utilized HRP immobilized on the novelnsupport and the in situ production of hydrogen peroxide to treat 4-bromophenol as a modelnsubstrate. The system was operated for almost 17 hours with no apparent decline in activity.