A Microscope system with a dual-band fil ter for The simultaneous enumeration of Cryptosporidium Parvum Oocysts and Sporozoites

摘要

A dual band filter set was designed to allow the simultaneous viewing of two fluoro- chromes (fluorescein isothiocyanate (FITC) and 4', 6-diamidino-2-phenylindole (DAPI)) so that Cryptosporidium oocysts and sporozoites can be viewed together. Simultaneous viewing increases Accuracy and decreases examination time as compared to current Environmental Protection Agency (EPA) Method 1623 for the detection of Cryptosporidium and Giardia in water by filtration/IMS/FA (immunomagnetic Separation/Fluorescent Antibodies).