Effect of salinity and inorganic nitrogen concentrations on nitrification and denitrification rates in intertidal sediments and rocky biofilms of the Douro River estuary, Portugal

摘要

The regulatory effects of salinity and inorganic nitrogen compounds on nitrification and denitrification were studied in intertidal sandy sediments and rocky biofilms in the Douro River estuary, Portugal, over a 12-month period. Nitrification and denitrification rates were measured in slurries of field samples and enrichment experiments using the difluoromethane and the acetylene inhibition techniques, respectively. Salinity did not regulate denitrification in either environment, suggesting that halotolerant bacteria dominated the denitrifier communities. However, nitrification rates were stimulated when salinity increased from 0 to 15 practical salinity units. NO_3~- addition experiments revealed that NO_3~- availability stimulates denitrification rates in sandy sediments, but not in rocky biofilms; however, in rocky biofilms a positive and linear relationship was observed between denitrification rates and water column NO_3~-concentrations (r = 0.92) during the monthly surveys. The N_2O:N_2 ratios increased rapidly when NO_3~- increased from 63 to 363 μM; however, results from monthly surveys showed that environmental parameters other than NO_3~-availability may be important in controlling the variation in N_2O production via denitrification. Ammonium additions to sandy sediments stimulated nitrification rates by 35% for the 20 μM NH_4~+ addition, but NH_4~+ appeared to inhibit nitrification at high concentration addition (200 μM NH_4~+). In contrast, rocky biofilm nitrification was stimulated by 65% when 200 μM NH_4~+ was added.