Sulfidogenesis process to strengthen re-granulation for biodegradation of methanolic wastewater and microorganisms evolution in an UASB reactor

摘要

A lab-scale methanolic wastewater-fed (3000 mg COD L-1) UASB reactor was operated for 235 days to evaluate the influence of the sulfidogenesis process on metabolic routes, the re-granulation of dispersed granules and long-term process performance. Various sulfidogenesis scenarios were created by stepwise decreasing the influent COD/SO42- ratio from 20 to 0.5 at a fixed organic loading rate (OLR) of 12 g COD L-1 d(-1). It was shown that the conversion of methanol to methane was stable at a wide COD/SO42- range of >= 2, attaining high biogas production rate of 3.78 +/- 0.32 L L-1 d(-1) with efficient concurrent removal of the total COD (96.5 +/- 4.4%) and sulfate (56.3 +/- 13.0%). The methane content in biogas remained relatively stable at 81.5 +/- 1.6% for all COD/SO42- ratios tested. The particle size of the granules was shown to clearly increase as the COD/SO42- ratios decreased. A slight linear decline was noted in the number of electrons utilized by methane producing archaea (MPA) (from 98.5 +/- 0.5% to 80.0 +/- 2.4%), whereas consumption by sulfate reducing bacteria (SRB) increased (from 1.5 +/- 0.5% to 20.0 +/- 2.4%) with the decreasing COD/SO42- ratio. According to the results of activity tests and microbial community analysis, the conversion of methanol to methane at a low COD/SO42- ratio, except from Methanomethylovorans sp., depends not only on low levels of acetoclastic and hydrogenotrophic methanogens, but also on incomplete oxidizer SRB species (e.g. Desulfovibrio sp.) that utilize H-2-CO2 with acetate to mineralize the methanol. This serves to diversify the metabolic pathway of methanol. Further analysis through scanning electron microscopy (SEM) revealed that a lower COD/SO42- ratio favored the sulfidogenesis process and diversified the microbial community inside the reactor. The benefical sulfidogenesis process subsequently invoked the formation of a sufficient, rigid [-Fe-EPS-](n) network (EPS: extracellular polymeric substances), binding and immobilizing the sludge, and resulting in the re-granulation of the dispersed granules. (C) 2016 Elsevier Ltd. All rights reserved.