DNA interactions of ruthenium(II) complexes with a polypyridyl ligand: 2-(2, 5-dimethoxyphenyl)-1H-imidazo[4,5-f]1,10-phenanthroline

摘要

This article describes the synthesis of a polypyridyl ligand, namely 2-(2, 5-dimethoxyphenyl)-1H-imidazo[4,5-f]1,10-phenanthroline (DMPIP) and its Ru(II) complexes, namely [Ru(bipy)₂DMPIP]2+ (1), [Ru(dmb)₂DMPIP]2+ (2) and [Ru(phen)₂DMPIP]2+ (3) ((bipy = 2,2′-bipyridine, dmb = 4,4′-dimethyl-2,2′-bipyridine, phen = 1,10-phenanthroline). The complexes were characterized by elemental analysis, plus IR, 1H-NMR and 13C [1H]-NMR spectra. The interactions of the complexes with calf thymus DNA were investigated. The results indicate that the three complexes can intercalate into DNA. Under irradiation at 365 nm, all three complexes promote the photocleavage of plasmid pBR 322 DNA. Inhibitor studies suggest that singlet oxygen plays a significant role in the cleavage mechanism for the complexes.