Experimental Infection of Taura Syndrome Virus (TSV) to Pacific White Shrimp (Litopenaeus vannamei), Black Tiger Shrimp (Penaeus monodon) and Giant Freshwater Prawn (Macrobrachium rosenbergii)

摘要

Laboratory infectivity of Taura syndrome virus (TSV) to Pacific white shrimp (Litopenaeus vannamei), black tiger shrimp (Penaeus monodon) and giant freshwater prawns (Macrobrachium rosenbergii) was investigated. All the infected L. vannamei died, while some P. monodon and M. rosenbergii survived. However, RT-PCR and in situ hybridization revealed TSV-positive results from surviving P. monodon and M. rosenbergii. Histopathological changes were observed in the subcuticular epidermis of infected L. vannamei. Extensive necrosis with prominent nuclear pyknosis and karyorrhexis of membranous tissues, including the abdominal segments and hindgut, were observed. Histopathological changes in P. monodon showed necrosis in the cuticular epithelium of the body surfaces; some of the infected cells showed pyknotic nuclei and melanization in the subcuticular layer tissues. In M. rosenbergii no histopathological changes of the cuticular epithelial layer were observed, only striated muscle cell necrosis.