A kinetic study of analyte-receptor binding and dissociation for biosensor applications: a fractal analysis for cholera toxin and peptide-protein interactions

摘要

A fractal analysis is presented for (a) analyte-receptor binding and (b) binding and dissociation kinetics for biosensor applications. Examples analyzed include the binding of different concentrations of cholera toxin (CT) in solution to fluorophore-labeled ganglioside GMl Immobilized on a biomimetic membrane surface [16], and tyrosine kinase lck SH2 domain in solution to a phosphorylated peptide Immobilized on a new cuvette-based surface plasmon resonance (SPR) instrument [14]. A single and a dual-fractal analysis is required to Describe the binding kinetics for CT.