Electrochemical genosensor based on modified octadecanethiol self-assembled monolayer for Escherichia coli detection

摘要

An electrochemical genosensor based on l-fluoro-2-nitro-4-azidobenzene (FNAB) modified octadecanethiol (ODT) self-assembled monolayer (SAM) has been fabricated for Escherichia coli detection. The results of electrochemical response measurements investigated using methylene blue (MB) as a redox indicator reveal that this nucleic acid sensor has 60s of response time, high sensitivity (0.5 × 10-18 M) and linearity as 0.5 × 10-18-1 ×10-6 M. The sensor has been found to be stable for about four months and can be used about ten times. It is shown that water borne pathogens like Klebsiella pneumonia. Salmonella typhimurium and other gram-negative bacterial samples has no significant effects in the response of this sensor.