A ratiometric colorimetric and fluorescent chemosensor for rapid detection hydrogen sulfide and its bioimaging

摘要

A new selective colorimetric and fluorescent sensor for H_2S, diethylaminocoumarin-hemicyanine dye has been designed, synthesized and evaluated. In CH_3OH-HEPES (10mM, pH 7.4) buffer solution containing the sensor, the addition of H_2S made the UV-vis absorption of the sensor taking place blue-shift from red to yellow, and the fluorescence of the sensor also changed from red to green. Other analytes did not disturb the determination of H_2S. The mechanism is based on the nucleophilic attack toward the polarized C=N and an electron-poor C=C bond of the sensor. Moreover, its adduct was proved by ESI-MS and NMR. The system was used to react with H_2S in the cell, which can provide a potentially powerful approach for probing H_2S chemistry in biological system.