A novel fluorometric turn-on assay for lipase activity based on an aggregation-induced emission (AIE) luminogen

摘要

Lipase plays critical roles in the industrial catalysis, drug discovery and medical diagnosis of diseases. In this paper, based on the consideration that a tetraphenylethylene (TPE) derivative P1 with a -COOC_6Hn group can be hydrolyzed into TPE-COOH which shows low solubility in same solutions and thus aggregation can occur easily, a novel fluorescence turn-on probe bearing aggregation-induced emission (AIE) characteristics to rapidly visualize lipase activities has been constructed with compound P1. Lipase of concentrations as low as 0.1 mgmL~(-1) can be detected and the assay range of lipase was located in 0.1-1.3 mgmL~(-1). On the other hand, the selectivity of probe P1, dynamic monitoring of enzymatic reaction and preliminary commercially enzyme activity screening was also summarized.