Layering of a film of carboxymethyl-botryosphaeran onto carbon black as a novel sensitive electrochemical platform on glassy carbon electrodes for the improvement in the simultaneous determination of phenolic compounds

摘要

In this work, a modification of a glassy carbon electrode (GCE) is presented for the first time using the polysaccharide carboxymethyl-botryosphaeran (CMB) and nanostructured carbon black (CB). The combination of the carbohydrate biopolymer and the carbonaceous nanomaterial (CMB-CB/GCE) showed improvements in the voltammetric responses of dopamine (DOP) and paracetamol (PAR) when compared to unmodified GCE. The morphology of the proposed sensor was characterized by scanning electron microscopy. The electrochemical characterization was performed by cyclic voltammetry and electrochemical impedance spectroscopy using the redox pair [Fe(CN)(6)](3-/4-). Using phosphate buffer solution (pH 7.0) and differential pulse voltammetry, DOP and PAR were determined simultaneously in the concentration range 0.099-2.9, and 0.70-19 mu mol L-1, with limits of detection of 0.013, and 0.11 mu mol L-1, respectively. The developed method with the fabricated electrochemical sensor is simple, fast, and sensitive. The proposed sensor was successfully applied to the individual and simultaneous determination of DOP and PAR in various samples, showing the practical feasibility of the proposed sensor as a novel platform for the sensitive determination of these phenolic compounds.