ELECTROCOMPETENT CELLS

摘要

CloneCatcher Gold DH5G Electrocompent E. coli cells exhibit extremely high electroporation efficiencies that approach the theoretical maximum of 3.4 x 1011 cfu/μg pUC19 DNA. Transferring exogenous DNA into E. coli is a standard laboratory method for cloning genes and constructing cDNA, genomic, and epitope libraries. The limiting factor in many library-screening efforts or multiple fragment ligations is the efficiency by which DNA can be introduced into E. coli. Electroporation is one method to efficiently introduce DNA into E. coli.