REAL-TIME PCR DETECTION SYSTEM

摘要

The CFX Connect real-time polymerase chain reaction (PCR) detection system is an ideal tool for researchers looking for reliable, high performance real-time PCR on a limited budget. The CFX Connect system, which requires a computer for operation, offers the same thermal performance as the CFX96 Touch real-time PCR system. It uses a similar scanning optics shuttle design that reads each well individually with high sensitivity and no cross talk to deliver optimal quantitative results. The CFX Connect detects two fluoro-phores per well in duplex experiments and detects SYBR Green or EvaGreen in singleplex experiments. Additional key benefits are effortless optimization that saves time and reduces costs by optimizing assays in a single run using the thermal gradient, and accurate data analysis with CFX Manager Software that offers an intuitive user interface and the ability to quickly and accurately validate and analyze data.