Toxicological responses of Carassius auratus induced by benzophenone-3 exposure and the association with alteration of gut microbiota

摘要

Gut microbiota plays a fundamental role in host's physiology. However, the effect of organic UV filters, an emerging contaminant on gut microbiota is poorly understood. Here, fish (Carassius auratus) were exposed to 2.20 and 200 ug/L of benzophenone-3 (BP3) for 28 days to explore the toxicological effects and its association with the changes in the gut microbiota. The BP3 accumulation is time and dose dependent in the liver and intestine. Under BP3 subchronic exposure, fish's body and intestinal weights, reactive oxygen species (ROS), immunoglobulin M (IgM) and vitellogenin (VTG) levels, as well as 7-benzyloxy-4-trifluoromethylcoumarin-0-debenzyloxylase (BFCOD) activities, were decreased. BP3 exposure has increased the abundance of Bacteroidetes phylum and Mycobacterium genus. Bioinformatic analysis revealed that the levels of ROS, IgM, estrogen receptor and VTG, activities of lysozyme, BFCOD and 7-ethoxyresorufin-O-deethylase were significantly correlated with the relative abundance of intestinal microbial genus (p < 0.05). These results highlight for the first time the association between the effects of organic UV filters on the antioxidant, immune, endocrine, and metabolic systems of the fish and changes in the gut microbiota, which extend knowledge of the role of gut microbiota in ecotoxicology.