Replication of cyclobutane pyrimidine dimer analogue by Ex Taq DNA polymerase

摘要

We previously reported an efficient and reversible template-directed photoligation using 5-carboxyvinyl-2′-deoxyuridine (~(CF)U)-containing ODN at the 5′-terminal. This method forms d(T-~(CV)U) as a cyclobutane pyrimidine dimer (CPD) analogue between the 3′-terminal thymidine and the 5′-terminal ~(CV)U of two oligodeoxynucleotides (ODNs). In this study, we performed PCR using a DNA template containing d(T-~(CV)U). Then, we found that two adenines were incorporated opposite the d(T-~(CV)U).