Natural killer cell cytotoxicity assay with time-resolved fluorimetry

摘要

A new time-resolved fluorimetric method for the measurement of natural killer (NK) cell cytotoxicity has been developed by labelling the target cell K562 with a new synthesized fluorescence marker KLUK. The method has advantages of higher sensitivity,time-saving, good reproducibility and has no radioactivity problems. A satisfactory result is obtained by comparing it with ~(51)Cr release method. It demonstrates that the new marker provides an alternative to currently used radioactive markers for theassessment of in vitro cellular cytotoxicity.