Regeneration of foreign genes co-transformed plants of Medicago sativa L by Agrobacterium rhizogenes

摘要

Gene encoding sulphur amino acid-rich protein (HNP) and rol genes were transferred into Medicago sativa L (alfalfa) mediated by Agrobacterium tumafeciens. Regeneration of trans- genic plants was induced successfully from hairy root tissue of cotyledon in alfalfa. Cotyledon tis- sues were an ideally transformed recipient. There was a negative correlation between age of hairy roots and embryogenesis frequency in alfalfa. Production of co-transformed plants with greater yield and supper quality was important for development of new alfalfa varieties.