Synthesis, Mechanical, In Vitro and In Vivo Bioactivity and Preliminary Biocompatibility Studies of Bioglasses

摘要

Nano beta-tricalcium phosphate scaffolds (beta-TCP) with MgO oxide were estimated in the bioglasses 40P(2)O(5)20Na(2)O- 10Ca(OH)(2)-20CaCl(2)-8.0ZnO-2.0MgO (BGMg) to improve that the mechanical, structural and biological properties. X-ray diffraction analysis (XRD) indicated that the major phase of beta-TCP and few phases of beta-TCP were investigated. The Vicker's microhardness, H-v, of prepared bioglasses was evaluated. Herein the antimicrobial activity, anti-proliferative/cytotoxicity against normal and activated splenic cells in vitro, and in vitro cytotoxicity effects and in vitro effects of activated splenic cells on BGMg. The results demonstrated that both nano (beta-TCP) with MgO particle attributed to antimicrobial activities against Gram negative and positive bacteria as well as fungi. It was obtained that the nanoparticles of (beta-TCP) were the stronger effect on the antimicrobial activity than the powder (beta-TCP). In addition (beta-TCP nanoparticles) demonstrated the cytotoxic effect on normal splenic cells and the prepared bioglasses BGMg did not cause any acute cytotoxicity or lysis to RBCs. Hence, addition MgO to the present glasses matrix P2O5-Na2O-Ca(OH)(2)-CaCl2-ZnO leads to optimize and enhancing biological and mechanical properties.