Optimization of the Production of a β-1, 4-endoglucanase from a Newly Isolated Bacillus sp.RL1 by Medium Optimization and Analysis Different Growth Parameters

摘要

The aim of this study was for the production of extracellular endoglucanase by a novel new strain of Bacillus sp. RL1 isolated from soil was improved by medium optimization. A low cost source of p-l, 4-Endoglucanase (EG) with robust activity is of potential commercial value. Here, we identified a soil bacterial strain, Bacillus sp. RL1, as a potential source for EG with high temperature tolerance. The culture parameters, such as duration of incubation, incubation temperature, pH, carbon and nitrogen sources and agitation speed and additives, were optimized for enhancing EG yield. The optimal level of each parameter for maximum EG production by Bacillus sp. RL1 was determined. Results showed that the EG production was higher 4-folds with pineapple containing production medium, beef extract, shaking 200 rpm and CoCL_2 and the incubation temperature, time course and pH were 35℃, 3rd day and 7.0 respectively, coparasion with basil medium (CMC). Thermal stability of EG was approximately 85.5% at 90℃ for 30 min. The enzyme maintained stability over a wide range of pH from 4-10. In addition optimized medium containing agricultural wastes pineapple combined with best two nitrogen sources (organic and inorganic nitrogen sources) showed significant activation on EG, FPase, glucosidase and xylanase were 18-folds, 6.5 -folds, 14- folds and 13- folds, respectively.