A Study of Non-Covalent Interaction between DNA-Binding Drugs and Double-Stranded Oligodeoxynucleotides By ESI Ion Trap Mass Spectrometry

摘要

We developed an assay method that utilizes ESI and ion trap mass spectrometry to rapidly determine the binding nature of drugs with oligodeoxynucleotides and to assess their relative affinities, stoichiometries and specificities in non-covalent interactions. Selectivity experiments show that the drugs H_2TM_pyP and CuTMpyP bind via mixed modes, whereasFeTMpyP and MnTMpyP interact by groove binding only.