Crystallization and Preliminary X-Ray Studies of the DNA-Binding Domain of Hepatocyte Nuclear Factor-6α Complexed with DNA

摘要

Hepatocyte nuclear factor 6 (HNF-6)/OC-1, a part of liver-enriched transcription factor, controls pancreas and liver development and regulates expression of several hepatic genes. DNA-binding region of HNF-6α bound to a 14-mer DNA fragment has been crystallized by the hanging drop vapor diffusion method. The crystals belong to space group P2 with unit cell parameters of a = 73.0 Å, b = 39.0 Å, c = 106.5 Å, β= 107.6 . X-ray diffraction data were collected to 2.0 Å resolution.