Design of a new rotating drum bioreactor for ligninolytic enzyme production by Phanerochete chrysosporium grown on an inert support

摘要

The production of ligninolytic enzymes by the white-rot fungus Phanerochaete chrysosporium BKM-F-1767 was studied in a new bioreactor configuration based on a standard rotating drum bioreactor. P chrysosporium was grown on cubes of nylon sponge, and cultivation was carried out in batch. Two aeration levels: 0.5 and 1 vvm were tested. The latter led to activities about 3-fold higher than the former, achieving maximum manganese-dependent peroxidase (MnP) and lignin peroxidase (LiP) activities of 1350 and 364 U/l, respectively.