Growth of the fungus Paecilomyces lilacinus with n-hexadecane in submerged and solid-state cultures and recovery of hydrophobin proteins

摘要

The filamentous fungus Paecilomyces lilacinus was grown on n-hexadecane in submerged (SmC) and solid-state (SSC) cultures. The maximum CO_2 production rate in SmC (V_(max) = 11.7 mg CO_2 L_g~(-1) day~(-1)) was three times lower than in SSC (V_(max) = 40.4 mg CO_2 L_g~(-1) day~(-1)). The P. lilacinus hydrophobin (PLHYD) yield from the SSC was 1.3 mg PLHYD g protein~(-1), but in SmC, this protein was not detected. The PLHYD showed a critical micelle concentration of 0.45 mg mL~(-1). In addition, the PLHYD modified the hydrophobicity of Teflon from 130.1 ± 2° to 47 ± 2°, forming porous structures with some filaments ＜1 μm and globular aggregates ＜0.25 μm diameter. The interfacial studies of this PLHYD could be the basis for the use of the protein to modify surfaces and to stabilize compounds in emulsions.