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Mitotic chromosomes are chromatin networks without a mechanically contiguous protein scaffold

机译:有丝分裂染色体是没有机械连续蛋白支架的染色质网络

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Isolated newt (Notophthalmus viridescens) chromosomes were studied by using micromechanical force measurement during nu-clease digestion. Micrococcal nuclease and short-recognition-sequence blunt-cutting restriction enzymes first remove the native elastic response of, and then to go on to completely disintegrate, single metaphase newt chromosomes. These experiments rule out the possibility that the mitotic chromosome is based on a mechanically contiguous internal non-DNA (e.g., protein) "scaffold"; instead, the mechanical integrity of the metaphase chromosome is due to chromatin itself. Blunt-cutting restriction enzymes with longer recognition sequences only partially disassemble mitotic chromosomes and indicate that chromatin in metaphase chromosomes is constrained by isolated chromatin-crosslinking elements spaced by ≈5 kb.
机译:分离的digest(Notophthalmus viridescens)染色体是通过在核酸酶消化过程中使用微机械力测量来研究的。微球菌核酸酶和短识别序列平切限制酶首先去除单个中期new的染色体的天然弹性反应,然后继续完全分解。这些实验排除了有丝分裂染色体基于机械连续的内部非DNA(例如蛋白质)“支架”的可能性。相反,中期染色体的机械完整性归因于染色质本身。具有较长识别序列的平切切割限制性内切酶只能部分分解有丝分裂染色体,表明中期染色体中的染色质受到间隔约5 kb的孤立染色质交联元件的约束。

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