Intra- and intermolecular translocation of the bi-domain transcription factor Oct1 characterized by liquid crystal and paramagnetic NMR

摘要

To perform their function as switches that control the expression of genes, transcription factors must efficiently locate their specific targets within a sea of DNA. Three mechanisms are thought to facilitate this search process: sliding, jumping, and intersegment transfer (1). In sliding, the transcription factor protein diffuses in one dimension along the DNA. Jumping involves the complete dissociation of the protein from the DNA, followed by 3D diffusion in free solution, and reassociation of the protein at a different site on the DNA.