Surface blebs on apoptotic cells are sites of enhanced procoagulant activity: Implications for coagulation events and antigenic spread in systemic lupus erythematosus

摘要

The restriction of phosphatidylserine (Ptd- Ser) to the inner surface of the plasma membrane bilayer is lost early during apoptosis. Since PtdSer is a potent surface procoagulant, and since there is an increased incidence of coagulation events in patients with systemic lupus erythe- matosus (SLE) who have anti-phospholipid antibodies, we addressed whether apoptotic cells are procoagulant and whether anti-phospholipid antibodies influence this. Apopto- tic HeLa cells, human endothelial cells, and a murine pre-B- cell line were markedly procoagulant in a modified Russell viper venom assay.