A combined kinetic and modeling study of the catalytic center subsites of human angiogenin

摘要

Kinetic analysis and molecular modeling have been used to map the ribonucleolytic center of angiogenin (Ang). Pyrimidine nucleotides were found to interact very weakly with Ang, consistent with the inaccessible B1 pyrimi- dine binding site revealed by x-ray crystallography. Ang also lacks an effective phosphate binding site on the 5' side of B_1.