Myosin dynamics in live Dictyostelium cells

摘要

Conventional myosin plays a key role in the cytoskeletal reorganization necessary for cytokinesis, migra- tion, and morphological changes associated with development in nonmuscle cells. We have made a fusion between the green fluorescent protein (GFP) and the Dictyostelium discoideum myosin heavy chain (GFP-myosin). The unique Dictyostelium system allows us to test the GFP-tagged myosin for activity both in vivo and in vitro.