Murine hematopoietic reconstitution after tagging and selection of retrovirally transduced bone marrow cells

摘要

A major problem facing the effective treat- ment of patients with cancer is how to get the specific antitumor agent into every tumor cell. In this report we describe the use of a strategy that, by using retroviral vectors encoding a truncated human CD5 cDNA, allows the selection of only the infected cells, and we show the ability to obtain, before bone marrow transplantation, a population of 5- fluouraci-treated murine bone marrow cells that are 100/100 marked. This marked population of bone marrow cells is able to reconstitute the hematopoietic system in lethally irradiated mice, indicating that the surface marker lacks deleterious effects on the functionality of bone marrow cells. No gross abnormalities in hematopoiesis were detected in mice repop- ulated with CD5-expressing cells.