Hemato-lymphoid in vivo reconstitution potential of subpopulations derived from in vitro differentiated embryonic stem cells

摘要

During differentiation in vitro, embryonic stem (ES) cells generate progenitors for most hemato-lymphoid lin- eages. We studied the developmental potential of two ES cell subpopulations that share the fetal stem cell antigen AA4.1 but differ in expression of the lymphoid marker B220 (CD45R). Upon transfer into lymphoid deficient mice, the B220~+ popula- tion generated a single transient wave of IgM~+ IgD~+ B cells but failed to generate T cells. In contrast, transfer of the B220~- fraction achieved long-term repopulation of both T and B lym- phoid compartments and restored humoral and cell-mediated immune reactions in the recipients.