Fertilization antigen-1: cDNA cloning, testis-specific expression, and immunocontraceptive effects

摘要

cDNA encoding for a sperm antigen, desig- nated fertilization antigen (FA-1), was cloned and sequenced from murine testis cDNA-λgt11 expression library using FA-1 mAb. Computer-generated translation analysis of 649-bp cDNA yielded an ORF of 164 amino acids with the first ATG Met start codon at nucleotide 81 and the stop codon TAA at nucleotide 577 and a polyadenylylation tail following the stop codon. The translated protein has a calculated molecular mass of 18.2 kDa and a potential N-linked glycosylation site at amino acids 158-160, besides at least two O-linked glyco- sylation sites.