METHOD OF PREPARATION OF CLONING SYSTEM cDNA FOR TRANSFORMATION AND EXPRESSION cDNA OF OVALBULINE OF COTURNIX JAPONICA IN PLANTS

摘要

From vector pT4, containing cDNA is gained the fragment of of cDNA with partially splitting fragment cDNA, the gained fragment is after isolation cloned in to cloning place of plasmide PA 35, containing the promoter of mosaic virus of cauliflower CaMV35 S with dimension 560 bp and noncoding area of nopaline gene NOP, by origin of vector pPA 35 characterized by splitting with specific restrictive endonucleases, from vector pPA35 is by splitting with Eco RI and Hind III the express cassette CaMV 35 S-cDNA -NOP is splitedit is cloned in to Eco RI and Hind III of cloning place of plasmide pDE 1001, containing the gene for resistance to kanamicine and the cells of E. coli are transformed with gained vector from this cells vector pPDE 1001, containing express cassette cDNA and is isolated and integrated with pertaining carrier.