DIRECT PHASE DETERMINATION IN PROTEIN ELECTRON CRYSTALLOGRAPHY - THE PSEUDO-ATOM APPROXIMATION

摘要

The crystal structure of halorhodopsin is determined directly in its centrosymmetric projection using 6.0-Angstrom-resolution electron diffraction intensities, without including any previous phase information from the Fourier transform of electron micrographs, The potential distribution in the projection is assumed a priori to be an assembly of globular densities. By an appropriate dimensional re-scaling, these ''globs'' are then assumed to be pseudo-atoms for normalization of the observed structure factors. After this treatment, the structure is determined directly by conventional direct methods, followed by Fourier refinement, leading to a mean phase deviation of only 20 degrees (from the values originally found from the image transform) for the 45 most intense reflections. [References: 24]