PU.1 can participate in an active enhancer complex without its transcriptional activation domain

摘要

The transcription factor PU.1 is necessary for the development of multiple hematopoietic lineages and contributes to the activity of the immunoglobulin k 3' en- hancer. A variety of proteins bind to the 3' enhancer (PU.1, PIP, ATF1, CREM, c-Fos, c-Jun, and E2A), but the mechanism of 3'-enhancer activity and the proteins necessary for its activity are presently unclear. We show here that PU.1 par- ticipates with other transcription factors in forming a higher- order complex with 3'-enhancer DNA sequences. Each protein is necessary for formation of this complex.